Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of corilagin on cerebral ischaemia/reperfusion-induced cerebral injury in a rat model

Feng Xu¹, Ai-Xia Li², Xin Li³, Bao Hui Liu⁴, Dong Ruan¹

¹Department of Neurosurgery, Xianning Central Hospital, The First Affiliated Hospital of Hubei University of Science and Technology; ²Department of Encephalopathy, Xianning Hospital of Traditional Chinese Medicine; ³Computer Science and Technology College, Hubei Science and Technology University, Xianning 437100; ⁴Department of Neurosurgery, Renmin Hospital, Wuhan University, Wuhan 430060, Hubei Province, China.

For correspondence:- Dong Ruan Email: DaiseyCeszie@yahoo.com Tel:+867158896172

Accepted: 19 November 2017 Published: 29 December 2017

Citation: Xu F, Li A, Li X, Liu BH, Ruan D. Effect of corilagin on cerebral ischaemia/reperfusion-induced cerebral injury in a rat model. Trop J Pharm Res 2017; 16(12):2885-2893 doi: 10.4314/tjpr.v16i12.12

© 2017 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To evaluate the neuroprotective effects of corilagin in cerebral ischaemia-induced cerebral injury in a rat model.

Methods: Cerebral ischaemia was induced by middle cerebral artery occlusion (MCAO). The animals were separated into five groups, including a control group that underwent surgery without inserting a monofilament; MCAO group that received saline; corilagin-treated group (20 mg/kg corilagin, intraperitoneally for 7 days after reperfusion); Tat-Beclin-1-treated group (intraperitoneal injection of 1.5mg/kg of Tat-Beclin-1 on the 3rd and 6th days after MCAO); and corilagin + Tat-Beclin-1-treated group (corilagin for 7 days and Tat-Beclin-1 on the 3rd and 6th days after MCAO). At the end of the treatments, neurological deficit, brain oedema, and volume of infarct were determined in all the animals. Moreover, the level of autophagy in infarcted tissues was evaluated by immunofluorescence, real-time PCR, and western blotting.

Results: There was a significant decrease in neurological deficit, brain oedema, and volume of infarcted tissue in corilagin-treated group when compared with MCAO- and Tat-Beclin-1-treated groups. Treatment with corilagin attenuated the autophagy of astrocytes and neurons in cerebral infarcted tissue, as demonstrated by immunofluorescence, quantitative PCR, and western blotting data.

Conclusion: Corlagin has a protective effect against neuronal damage in cerebral ischaemic rats by decreasing neurological deficit score, infarct volume, and water content of cerebral tissue. Corlagin attenuates autophagy in cerebral tissue, thus protecting cerebral ischaemic rats from neuronal damage

Keywords: Corilagin, Cerebral ischaemia, Autophagy, Tat-Beclin-1, Neronal damage, Astrocytes, Neurological deficit score, Infarct volume